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Journal Article > Research

Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up

Schramm B, Hewison CCH, Bonte L, Jones W, Camelique O, Ruangweerayut R, Swaddiwudhipong W, Bonnet MMB
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Abstract
Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.
Countries
Thailand
Subject Area
tuberculosisdiagnosticsfilovirus
DOI
10.1128/JCM.01232-12
Published Date
30-May-2012
PubMed ID
22649015
Languages
English
Journal
Journal of Clinical Microbiology
Volume / Issue / Pages
Volume 50, Issue 8
Issue Date
01-Aug-2012
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