Journal Article > ResearchFull Text
J Microbiol Methods. 2009 May 7; Volume 78 (Issue 1); 107-108.; DOI:10.1016/j.mimet.2009.05.001
Martin AIC, Fissette K, Varaine FFV, Portaels F, Palomino JC
J Microbiol Methods. 2009 May 7; Volume 78 (Issue 1); 107-108.; DOI:10.1016/j.mimet.2009.05.001
We compared the sensitivity and time to detection of growth of Mycobacterium tuberculosis in the thin layer agar (TLA) compared to BACTEC MGIT960. The average time for growth of M. tuberculosis in TLA and BACTEC MGIT960 was 10.6 and 9.6 days, respectively. The sensitivity of detection of M. tuberculosis was 97.3% on TLA and 97% on BACTEC MGIT960 for smear positive samples. TLA showed comparable results to BACTEC MGIT960 and could be an alternative method for low-income countries.
Journal Article > ResearchFull Text
J Microbiol Methods. 2008 October 1; Volume 75 (Issue 2); DOI:10.1016/j.mimet.2008.06.015
Palomino JC, Martin AIC, Von Groll A, Portaels F
J Microbiol Methods. 2008 October 1; Volume 75 (Issue 2); DOI:10.1016/j.mimet.2008.06.015
Tuberculosis still represents a major public health problem, especially in low-resource countries where the burden of the disease is more important. Multidrug-resistant and extensively drug drug-resistant tuberculosis constitute serious problems for the efficient control of the disease stressing the need to investigate resistance to first- and second-line drugs. Conventional methods for detecting drug-resistance in Mycobacterium tuberculosis are slow and cumbersome. The most commonly used proportion method on Löwenstein-Jensen medium or Middlebrook agar requires a minimum of 3-4 weeks to produce results. Several new approaches have been proposed in the last years for the rapid and timely detection of drug-resistance in tuberculosis. This review will address phenotypic culture-based methods for rapid drug susceptibility testing in M. tuberculosis.