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Journal Article > Research

Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous Blood Samples

Canier L, Khim N, Kim S, Eam R, Khean C, Loch K, Ken M, Pannus P, Bosman P, Stassijns J, Nackers F, Alipon SC, Char MC, Chea N, Etienne W, de Smet M, Kindermans JM, Menard D
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Abstract
In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.
Countries
Cambodia
Subject Area
diagnosticsmalariafilovirus
DOI
10.4269/ajtmh.14-0614
Published Date
05-Jan-2015
PubMed ID
25561570
Languages
English
Journal
American Journal of Tropical Medicine and Hygiene
Volume / Issue / Pages
Volume 92, Issue 3, Pages 573-577
Issue Date
04-Mar-2015
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