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Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone | Journal Article / Research | MSF Science Portal
Journal Article
|Research

Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone

Park DJ, Dudas G, Wohl S, Goba A, Whitmer SL, Andersen KG, Sealfon RS, Ladner JT, Kugelman JR, Matranga CB, Winnicki SM, Qu J, Gire SK, Gladden-Young A, Jalloh S, Nosamiefan D, Yozwiak NL, Moses LM, Jiang PP, Lin AE, Schaffner SF, Bird B, Towner JS, Mamoh M, Gbakie M, Kanneh L, Kargbo D, Massally JL, Kamara FZ, Konuwa E, Sellu J, Jalloh AA, Mustapha I, Foday M, Yillah M, Erickson BR, Sealy T, Blau D, Paddock C, Brault AC, Amman B, Basile J, Bearden S, Belser JA, Bergeron E, Campbell S, Chakrabarti A, Dodd K, Flint M, Gibbons A, Goodman C, Klena J, McMullan L, Morgan L, Russell B, Salzer JS, Sanchez A, Wang D, Jungreis I, Tomkins-Tinch C, Kislyuk A, Lin MF, Chapman S, MacInnis B, Matthews A, Bochicchio J, Hensley L, Kuhn JH, Nusbaum C, Schieffelin JS, Birren BW, Forget M, Nichol ST, Palacios GF, Ndiaye D, Happi CT, Gevao SM, Vandi MA, Kargbo B, Holmes EC, Bedford T, Gnirke A, Stroher U, Rambaut A, Garry RF, Sabeti PC
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Abstract
The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.

Countries

Sierra Leone

Subject Area

Ebola

Languages

English
DOI
10.1016/j.cell.2015.06.007
Published Date
18 Jun 2015
PubMed ID
26091036
Journal
Cell
Volume | Issue | Pages
Volume 161, Issue 7
Issue Date
2015-06-01
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